![]() Introduction, fundamental modes of vibrations in polyatomic molecules, sample handling, factors affecting vibrations Theory, Concepts of singlet, doublet, and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation, and applications Instrumentation – Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photovoltaic cell, Silicon Photodiode.Īpplications – Spectrophotometric titrations, Single-component and multi-component analysis Fluorimetry UNIT I UV Visible spectroscopy, Fluorimetry UV Visible spectroscopyĮlectronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation, and deviations. UNIT V Ion exchange, Gel, Affinity Chromatography.UNIT III Introduction to chromatography. ![]() UNIT I UV Visible spectroscopy, Fluorimetry. ![]()
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